Journal: bioRxiv

Article Title: The selective OX1R antagonist 1-SORA-51 reduces binge-like feeding behavior in male and female mice without detectable changes in dopamine

doi: 10.64898/2026.04.24.720455

Figure Lengend Snippet: All C57BL/6J mice were pre-exposed to the high-fat diet for 48 hours with std. chow, and then intermittently exposed the high-fat diet (along with std. chow) for 24 hours each binge session. 1A, B) Food intake was assessed over 24 hours for both male (n=10) and female (n=10) mice for the day prior (chow only) to a binge session and then a 24-hour binge session (chow with the high-fat diet). Males and females displayed binge-like feeding behavior with highly significant increases in total kcal/g BW consumed during binge sessions compared to the chow-only consumption. C) A 2-hour timepoint (to match food intake measurements after treatments) revealed significant binge-like behavior compared to a chow-only session for males and females. D-G) No effect of DORA-22 (100 mg/kg) was observed on binge-like feeding at the 2- or 24-hour timepoints for both sexes (n= 9 males, 10 females). All error bars are S.E.M.

Article Snippet: The high-fat diet used for all studies (5TJN, TestDiet, Richmond, Indiana) provides 4.55 kcal/g with the following caloric breakdown: protein 15.8%, fat 39.1%, and carbohydrates 45.1%.

Techniques:

Journal: bioRxiv

Article Title: The selective OX1R antagonist 1-SORA-51 reduces binge-like feeding behavior in male and female mice without detectable changes in dopamine

doi: 10.64898/2026.04.24.720455

Figure Lengend Snippet: All C57BL/6J mice were pre-exposed to the high-fat diet for 48 hours with std. chow, and then intermittently exposed the high-fat diet (along with std. chow) for 24 hours each binge session. A) A significant reduction in binge-like feeding was observed at the 2-hour timepoint for male mice treated with 1-SORA-51 (100 mg/kg) compared to vehicle treatment sessions (vehicle n=10, 1-SORA-51 n=8). B) The effect of 1-SORA-51 did not last across the 24-hour period. C) Similarly, a significant reduction in binge-like feeding was observed at the 2-hour timepoint for female mice treated with 1-SORA-51 (100 mg/kg) compared to vehicle treatment sessions (vehicle n=8, 1-SORA-51 n=9). D) The effect of 1-SORA-51 did not last across the 24-hour period. All error bars are S.E.M.

Article Snippet: The high-fat diet used for all studies (5TJN, TestDiet, Richmond, Indiana) provides 4.55 kcal/g with the following caloric breakdown: protein 15.8%, fat 39.1%, and carbohydrates 45.1%.

Techniques:

Journal: bioRxiv

Article Title: The selective OX1R antagonist 1-SORA-51 reduces binge-like feeding behavior in male and female mice without detectable changes in dopamine

doi: 10.64898/2026.04.24.720455

Figure Lengend Snippet: A) Male and female mice (n=9) expressing the GRABDA 2m sensor and finished binge training underwent fiber photometry sessions, during which baseline was recorded prior to treatment via gavage, and then provided access to the high-fat diet for 2 hours. Shown here is fluorescence normalized to baseline. No effect of treatment was seen during the 30 minutes after gavage or after HF pellet delivery. B) Treatment with 1-SORA-51 resulted in significantly less high-fat pellet consumption compared to vehicle sessions. All error bars are S.E.M.

Article Snippet: The high-fat diet used for all studies (5TJN, TestDiet, Richmond, Indiana) provides 4.55 kcal/g with the following caloric breakdown: protein 15.8%, fat 39.1%, and carbohydrates 45.1%.

Techniques: Expressing, Fluorescence